Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13588] to SAFB - C-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SAFB antibody [EPR13588] - C-terminal
    See all SAFB primary antibodies

  • Description

    Rabbit monoclonal [EPR13588] to SAFB - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and A431 cells, SH SY5Y and 293 cell line lysates, mouse heart tissue; Human and rat brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13588

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Western blot - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/20000 dilution

    Lane 1 : HeLa cell line lysate
    Lane 2 : SH SY5Y cell line lysate
    Lane 3 : 293 cell line lysate
    Lane 4 : A431 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Western blot - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat brain tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

    Immunohistochemical analysis of paraffin embedded rat brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).

  • Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)
    Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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