Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp90 alpha antibody [5G5] (ab128483)

Key features and details

  • Mouse monoclonal [5G5] to Hsp90 alpha
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hsp90 alpha antibody [5G5]
    See all Hsp90 alpha primary antibodies

  • Description

    Mouse monoclonal [5G5] to Hsp90 alpha

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human Hsp90 alpha expressed in E. coli.

  • Positive control

    • Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
      Flow Cyt: HeLa cells
      IHC-P: Lung cancer tissue

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution + Human Hsp90 alpha (amino acids 275-484) recombinant protein

    Predicted band size: 85 kDa



    Expected MWt of recombinant protein is 50.5 kDa.
  • ELISA - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    ELISA - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    ELISA using ab128483.
  • Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    All lanes : Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution

    Lane 1 : NIH 3T3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HCT116 cell lysate
    Lane 4 : HL-60 cell lysate
    Lane 5 : SK-Br-3 cell lysate
    Lane 6 : C6 cell lysate
    Lane 7 : COS7 cell lysate

    Predicted band size: 85 kDa

  • Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    ab128483, at a 1/200 dilution, staining Hsp90 alpha in HeLa cells by Flow Cytometry (green). Negative control is shown in red.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)
    ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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