Anti-Hsc70 antibody [1F2-H5] (ab223356)
Key features and details
- Mouse monoclonal [1F2-H5] to Hsc70
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-Hsc70 antibody [1F2-H5]
See all Hsc70 primary antibodies -
Description
Mouse monoclonal [1F2-H5] to Hsc70 -
Host species
Mouse -
Specificity
Does not cross react with HSP70. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human Hsc70.
Database link: P11142 -
Positive control
- WB: Mixture of 10 human cell lines; recombinant Hsc70. ICC/IF: HaCaT (human keratinocyte cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1F2-H5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/200 dilution. The nuclear counter stain is DAPI (blue) used at 1/40,000 dilution. The right-hand panel shows the merged images.
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All lanes : Anti-Hsc70 antibody [1F2-H5] (ab223356) at 1/1000 dilution
Lane 1 : Mixture of ten different human cell lines at 15 µg
Lane 2 : Recombinant Hsp72
Lane 3 : Recombinant Hsc70
Secondary
All lanes : Sheep anti-mouse IgG HRPPrimary incubation: 2 hours at room temperature.
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2% formaldehyde-fixed heat-shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (yellow) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: Goat Anti-Mouse at 1/200. The nuclear counter stain is DAPI at 1/40,000 (blue). The right-hand panel shows the merged images.
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Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/50 dilution.