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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsc70 antibody [1F2-H5] (ab223356)

Anti-Hsc70 antibody [1F2-H5] (ab223356)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1F2-H5] to Hsc70
  • Suitable for: ICC/IF, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Hsc70 antibody [1F2-H5]
    See all Hsc70 primary antibodies
  • Description

    Mouse monoclonal [1F2-H5] to Hsc70
  • Host species

    Mouse
  • Specificity

    Does not cross react with HSP70.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant full length protein corresponding to Human Hsc70.
    Database link: P11142

  • Positive control

    • WB: Mixture of 10 human cell lines; recombinant Hsc70. ICC/IF: HaCaT (human keratinocyte cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1F2-H5
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

    2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/200 dilution. The nuclear counter stain is DAPI (blue) used at 1/40,000 dilution. The right-hand panel shows the merged images.

  • Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)
    Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)
    All lanes : Anti-Hsc70 antibody [1F2-H5] (ab223356) at 1/1000 dilution

    Lane 1 : Mixture of ten different human cell lines at 15 µg
    Lane 2 : Recombinant Hsp72
    Lane 3 : Recombinant Hsc70

    Secondary
    All lanes : Sheep anti-mouse IgG HRP


    Primary incubation: 2 hours at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

    2% formaldehyde-fixed heat-shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (yellow) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: Goat Anti-Mouse at 1/200. The nuclear counter stain is DAPI at 1/40,000 (blue). The right-hand panel shows the merged images.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

    Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/50 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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