Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
![Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-389690-cdkn2c-257887-192239-full--anti-p18-ink4ccdkn2c-antibody-epr15891-western-blot-human-lysate.jpg "Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C
- Suitable for: WB, IP, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-p18 INK4c/CDKN2C antibody [EPR15891]
See all p18 INK4c/CDKN2C primary antibodies -
Description
Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB RatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEL-293T, Ramos, HeLa, Jurkat, Rat kidney, Rat spleen and NIH 3T3 lysates. IHC-P: Human brain and glioma tissues; ICC/IF: HeLa cells.
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General notes
This product was previously labelled as p18 INK4c
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15891 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDKN2C knockout HeLa cell lysate
Lane 3 : Rat Kidney cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDaLanes 1-4: Merged signal (red and green). Green - ab192239 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab192239 Anti-p18 INK4c/CDKN2C antibody [EPR15891] was shown to specifically react with Cyclin Dependent Kinase Inhibitor 2C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265031 (knockout cell lysate ab257887) was used. Wild-type and Cyclin Dependent Kinase Inhibitor 2C knockout samples were subjected to SDS-PAGE. ab192239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-253283-anti-p18-ink4c-antibody-epr15891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230025-anti-p18-ink4c-antibody-epr15891-western-blot.jpg)
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate
Lane 3 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa
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![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230029-anti-p18-ink4c-antibody-epr15891-immunoprecipitation.jpg)
Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230027-anti-p18-ink4c-antibody-epr15891-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230026-anti-p18-ink4c-antibody-epr15891-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230024-anti-p18-ink4c-antibody-epr15891-western-blot.jpg)
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa
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![Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-9-benefits-of-recombinant-antibodies.png)
Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
