Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP435Y] to CDKN2A/p16INK4a - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free
    See all CDKN2A/p16INK4a primary antibodies

  • Description

    Rabbit monoclonal [EP435Y] to CDKN2A/p16INK4a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CDKN2A/p16INK4a.

  • Positive control

    • Flow Cyt: HEK-293 cells IP: HeLa whole cell lysate.

  • General notes

    ab247292 is the carrier-free version of ab40803 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247292 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)
    Western blot - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)
    Anti-CDKN2A/p16INK4a antibody [EP435Y] (ab40803) at 1/2000 dilution + 293 cell lysate at 10 µg

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using ab40803, the same antibody clone in a different buffer formulation.

     

  • Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)
    Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)

    This data was developed using ab40803, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDKN2A/p16INK4a with purified ab40803 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)
    Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)

    This data was developed using ab40803, the same antibody clone in a different buffer formulation.
    Purified ab40803 at 1/50 dilution (2µg) immunoprecipitating CDKN2A/p16INK4a in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab40803 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40803 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 17 kDa

  • Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)
    Anti-CDKN2A/p16INK4a antibody [EP435Y] - BSA and Azide free (ab247292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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