Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Wednesday March 10, 2021

Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6528(N)] to p38 gamma/MAPK12
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Rat, Human

Product name

Anti-p38 gamma/MAPK12 antibody [EPR6528(N)]
See all p38 gamma/MAPK12 primary antibodies
Description

Rabbit monoclonal [EPR6528(N)] to p38 gamma/MAPK12
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle lysate; HEK-293T, HeLa, K562, C6 and PC-12 whole cell lysates; Rat heart, spleen and muscle lysates. ICC/IF: A673 and K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6528(N)
Isotype

IgG
Research areas

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MAPK12 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Lanes 1-3: Merged signal (red and green). Green - ab205926 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

ab205926 Anti-MAPK 12 antibody [EPR6528(N)] was shown to specifically react with MAPK 12 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266280 (knockout cell lysate ab258041) was used. Wild-type and MAPK 12 knockout samples were subjected to SDS-PAGE. ab205926 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A673 (Human muscle Ewing's Sarcoma cell line) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on A673 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

Lane 1: Wild type HAP1 whole cell lysate (0 µg)
Lane 2: empty knockout HAP1 whole cell lysate (20 µg)
Lane 3: MAPK 12 whole cell lysate (0 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab205926 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/10000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal skin lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

Lane 1 : Rat heart lysate
Lane 2 : Rat spleen lysate
Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : Rat muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 1 minute; Lane 5: 30 seconds
Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)

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