Anti-ORC4L antibody (ab9641)
Key features and details
- Goat polyclonal to ORC4L
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ORC4L antibody
See all ORC4L primary antibodies -
Description
Goat polyclonal to ORC4L -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide: DVRQWATSSLSWL, corresponding to C terminal amino acids 424-436 of Human ORC4L.
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General notes
Official Gene Symbol - ORC4L GenBank Accession Number – NP_002543 LocusLink ID - 5000
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg
Predicted band size: 50 kDa
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Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
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Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence. -
Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)All lanes : Anti-ORC4L antibody (ab9641) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
