Anti-APE1 antibody (ab235942)
Key features and details
- Rabbit polyclonal to APE1
- Suitable for: WB, IHC-P, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-APE1 antibody
See all APE1 primary antibodies -
Description
Rabbit polyclonal to APE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Chimpanzee, Gorilla -
Immunogen
Recombinant full length protein corresponding to Human APE1 aa 1 to the C-terminus.
Database link: P27695 -
Positive control
- WB: HeLa, MCF7, HepG2, Raji, A549, K562 and NIH/3T3 whole cell lysates; Mouse brain and liver lysates. IHC-P: Human lung, testis and tonsil tissues. ChIP: Chromatin prepared from MCF7 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 0.58% Sodium chloride -
Concentration information loading...
-
Purity
Caprylic Acid - Ammonium Sulfate precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-APE1 antibody (ab235942)
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 8 : Mouse liver lysate
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 35 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
Paraffin-embedded human tonsil tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
Paraffin-embedded human testis tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
Paraffin-embedded human lung tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.
-
ChIP analysis of chromatin prepared from 1.1 x 106 MCF7 (human breast adenocarcinoma cell line) cells. MCF7 were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg ab235942 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the MDR1 promoter.