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Anti-APE1 antibody (ab235942)

Anti-APE1 antibody (ab235942)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to APE1
  • Suitable for: WB, IHC-P, ChIP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-APE1 antibody
    See all APE1 primary antibodies
  • Description

    Rabbit polyclonal to APE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Chimpanzee, Gorilla
  • Immunogen

    Recombinant full length protein corresponding to Human APE1 aa 1 to the C-terminus.
    Database link: P27695

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa, MCF7, HepG2, Raji, A549, K562 and NIH/3T3 whole cell lysates; Mouse brain and liver lysates. IHC-P: Human lung, testis and tonsil tissues. ChIP: Chromatin prepared from MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Caprylic Acid - Ammonium Sulfate precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

Images

  • Western blot - Anti-APE1 antibody (ab235942)
    Western blot - Anti-APE1 antibody (ab235942)
    All lanes : Anti-APE1 antibody (ab235942)

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 7 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 8 : Mouse liver lysate
    Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)

    Paraffin-embedded human tonsil tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)

    Paraffin-embedded human testis tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody (ab235942)

    Paraffin-embedded human lung tissue stained for APE1 using ab235942 at 1/100 dilution in immunohistochemical analysis.

  • ChIP - Anti-APE1 antibody (ab235942)
    ChIP - Anti-APE1 antibody (ab235942)

    ChIP analysis of chromatin prepared from 1.1 x 106 MCF7 (human breast adenocarcinoma cell line) cells. MCF7 were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg ab235942 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the MDR1 promoter.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-APE1 antibody [EPR18378-45] - BSA and Azide free (ab227062)

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    Anti-APE1 antibody [EPR18378-45] - ChIP Grade (ab189474)

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