Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149)
Key features and details
- Rabbit polyclonal to Histone H3 (symmetric di methyl R8)
- Suitable for: ChIP, WB
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (symmetric di methyl R8) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (symmetric di methyl R8) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Caenorhabditis elegans
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (symmetric di methyl R8).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo lysate. ChIP: Chromatin prepared from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149)Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Western blot - Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149)All lanes : Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149) at 2 µg/ml
Lane 1 : R8Me2a synthetic peptide
Lane 2 : R8Me2s synthetic peptide
Lane 3 : R8Me1 synthetic peptide
Lane 4 : Histone H3 R8 synthetic peptide
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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ChIP - Anti-Histone H3 (symmetric di methyl R8) antibody (ab272149)Chromatin Immunoprecipitation of Histone H3 (symmetric di methyl R8) with ab272149. Chromatin from one million formaldehyde cross-linked HeLa (human epithelial cell line from cervix adenocarcinoma) cells with 2 µg of Anti-Histone H3 R8 me2a was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).