Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade
- Suitable for: WB, ICC/IF, ChIP
- Reacts with: Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (acetyl K12) antibody - ChIP Grade
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (acetyl K12) - ChIP Grade -
Host species
Rabbit -
Specificity
Ab46983 recognizes Histone H4 acetylated at lysine 12. Serum did not react with non-modified Histone H4 peptide or peptides acetylated at lysines 5, 8 or 16. -
Tested applications
Suitable for: WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae
Predicted to work with: a wide range of other species
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- IF: HeLa cells WB: Acid extract of HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituent: 30% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)
ab46983 tested by ChIP analysis. ChIP performed using Hela chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of ab46983 or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as fold enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)Staining of HeLa cells with ab46983 at a dilution of 1/500.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)ab46983 staining Hela cells at 1/500 (top image) and DAPI (bottom image).
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Western blot - Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983)All lanes : Anti-Histone H4 (acetyl K12) antibody - ChIP Grade (ab46983) at 1/10000 dilution
Lane 1 : Acid extract of
HeLa cells without treatment.
Lane 2 : Acid extract of
HeLa cells treated
with sodium butyrate.
Predicted band size: 11 kDa
Observed band size: 11 kDa
