Anti-Histone H3 (phospho T11) antibody (ab272152)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T11)
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho T11) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T11) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho T11).
Database link: Q71DI3 -
Positive control
- WB: HeLa histone preparation. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (phospho T11) antibody (ab272152)Anti-Histone H3 (phospho T11) antibody (ab272152) at 1/1000 dilution + HeLa histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T11) antibody (ab272152)Immunofluorescent analysis of 0.5% PFA-fixed HeLa cells labeling Histone H3 (phospho T11) using ab272152 at 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1:10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).
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Dot Blot - Anti-Histone H3 (phospho T11) antibody (ab272152)Dot Blot analysis of Histone H3 (phospho T11) using ab272152 at 1/1000 dilution.
Lane 1: Unmodified.
Lane 2: S10.
Lane 3: S10-T11.
Lane 4: T11.
Load: 0.1, 0.5, and 1.0 picomoles of peptide.