Beta Galactosidase Detection Kit (Fluorometric) (ab176721)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader, Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 0.3 mU/well
Overview
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Product name
Beta Galactosidase Detection Kit (Fluorometric)
See all beta galactosidase kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Sensitivity
>= 0.3 mU/well -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Beta Galactosidase Detection Kit (Fluorometric) (ab176721) uses the fluorogenic fluorescein digalactoside (FDG) galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates the strongly fluorescent fluorescein upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. FDG used in the kit is not fluorescent. The galactosidase induced cleavage of FDG gives fluorescein that has the spectra of Ex/Em = 490/515 nm, which can be detected with most fluorescence instruments equipped with a FITC filter set. The kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader or a fluorescence microscope. It might also be used for screening galactosidase inhibitors or inducers.
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Platform
Microplate reader, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Beta-mercaptoethanol 1 x 500µl DMSO 1 x 500µl Fluorescein di-β-D-Galactopyranoside (FDG) 1 vial Lysis Buffer 1 x 25ml Reaction Buffer 1 x 50ml Stop Buffer 1 x 25ml -
Research areas
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Relevance
Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity. -
Cellular localization
Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes. -
Alternative names
- Acid beta galactosidase
- Beta galactosidase 1
- EBP
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Images
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Beta Galactosidase Dose Response Curve
Beta Galactosidase dose response was measured with Beta Galactosidase Detection Kit (Fluorometric) (ab176721) in a black 96-well plate using a fluorescence microplate reader. As low as 0.3 mU Beta Galactosidase can be detected with 30 minutes incubation.
