beta Galactosidase Staining Kit (ab102534)
Key features and details
- Assay type: Direct
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
beta Galactosidase Staining Kit
See all beta galactosidase kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Product overview
The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.
Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.
Visit our FAQs page for tips and troubleshooting.
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Notes
This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 250 tests Fixative Solution (1X) NM 1 x 125ml Staining Solution (1X) WM 1 x 125ml Staining Supplement (100X) Red 1 x 1.5ml X-Gal, chromogenic beta-galactosidase substrate Green 1 x 150mg -
Research areas
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Relevance
Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity. -
Cellular localization
Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes. -
Alternative names
- Acid beta galactosidase
- Beta galactosidase 1
- EBP
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Images
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Beta Galactosidase Detection Kit (ab102534) Li XH et al., PLoS One, 7, e36604, 2012 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Gamma-radiation induced senescence in hFOB cells. The number of senescence-associated-β-galactosidase positive cells (left panel) cleary increases 72h after 8 Gy γ irradiation. Representative data was from one of a total of 3 indepent experiments. Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604
