Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)
![Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)](https://www.abcam.com/ps/products/42/ab42228/Images/ab42228-55612-anti-vegf-receptor-2-antibody-mv10013m-h-sandwich-elisa.jpg "Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)")
Key features and details
- Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-VEGF Receptor 2 antibody [mV1001.3m-h]
See all VEGF Receptor 2 primary antibodies -
Description
Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Purified fragment, corresponding to N terminal amino acids 30-200 of Human VEGF Receptor 2
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Positive control
- Human Umbilical Vein Endothelial Cells (HUVECs).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mV1001.3m-h -
Isotype
IgG1 -
Research areas
Images
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Sandwich ELISA - Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.
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![Western blot - Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)](https://www.abcam.com/ps/products/42/ab42228/Images/ab42228-55611-anti-vegf-receptor-2-antibody-mv10013m-h-western-blot.jpg)
Western blot - Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)Lane 1 : unstimulated cells
Lane 2 : hVEGF at 20 ng/ml
Lane 3 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 5 µg/ml
Lane 4 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 25 µg/ml
All lanes : HUVECs
Predicted band size: 151 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
HUVECs (human umbilical vein endothelial cells) were stimulated with hVEGF or ab42228 for 30min. Phospho-VEGF Receptor 2 was detected with IP-Western for Phosphotyrosine.
