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Anti-RCN1/RCN antibody [EPR19193] (ab184441)

Price and availability

247 259 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-RCN1/RCN antibody [EPR19193] (ab184441)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19193] to RCN1/RCN
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-RCN1/RCN antibody [EPR19193]
    See all RCN1/RCN primary antibodies
  • Description

    Rabbit monoclonal [EPR19193] to RCN1/RCN
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, heart, kidney and spleen lysates; HeLa, HEK-293, U-87 MG, C6, SH-SY5Y, Neuro-2a, RAW 264.7, PC-12 and NIH/3T3 cell lysates; E14 mouse and rat embryo lysates; Rat cerebral cortex, brain, heart and spleen lysates; Mouse brain, heart and spleen lysates.
  • General notes

     

     

     This product was previously labelled as RCN1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19193
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER

Images

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 30 seconds; Lane 4: 3 minutes.

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : E14 mouse embryo lysate
    Lane 5 : E14 rat embryo lysate
    Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 8 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 9 : Rat cerebral cortex lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:  Lane 1-6: 8 seconds; Lane 7 and 8: 30 seconds; Lane 9: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-6: 3 minutes Lane 7 and 8: 30 seconds; Lane 9 and 10: 1 second.

  • Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Anti-RCN1/RCN antibody [EPR19193] (ab184441)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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