Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-RCN1/RCN antibody [EPR17163] - C-terminal
See all RCN1/RCN primary antibodies
Description

Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: 293 cell lysate and HeLa cell lysate. IHC: Human cerebral cortex tissue. ICC/IF: HeLa cells. IP and Flow Cyt: HeLa cells.
General notes
This product was previously labelled as RCN1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17163
Isotype

IgG
Research areas

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution

Lane 1 : 293 (Human epithelial cells from embryonic kidney) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa
Observed band size: 44, 46 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and diluting buffer was 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 8416973.
Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution

Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa
Observed band size: 44, 46 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and diluting buffer was 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 8416973.
Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

RCN1/RCN was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

Lane 2: HeLa whole cell extract.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.

The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Flow cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

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