Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RCN1/RCN antibody [EPR17163] - C-terminal
    See all RCN1/RCN primary antibodies

  • Description

    Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293 cell lysate and HeLa cell lysate. IHC: Human cerebral cortex tissue. ICC/IF: HeLa cells. IP and Flow Cyt: HeLa cells.

  • General notes

     

     

     This product was previously labelled as RCN1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17163

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution

    Lane 1 : 293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 44, 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and diluting buffer was 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 44, 46 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and diluting buffer was 5% NFDM/TBST.
    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

  • Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    RCN1/RCN was immunoprecipitated from 1mg of HeLa  (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

    Lane 2: HeLa whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973. 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Flow Cytometry - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Flow Cytometry - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Flow cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)
    Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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