Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11149(2)] to KLC3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free
    See all KLC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11149(2)] to KLC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250220 is the carrier-free version of ab180523.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U-87 MG cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab180523, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    This data was developed using ab180523, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Immunocytochemistry - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    This data was developed using ab180523, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
  • Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    This data was developed using ab180523, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
  • Flow Cytometry - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Flow Cytometry - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    This data was developed using ab180523, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
  • Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
    Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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