Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MCAK antibody [EPR14838] (ab187652)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14838] to MCAK
Suitable for: Flow Cyt, ICC/IF, WB
Knockout validated
Reacts with: Human

Product name

Anti-MCAK antibody [EPR14838]
See all MCAK primary antibodies
Description

Rabbit monoclonal [EPR14838] to MCAK
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7, 293T, Hela and K562 cell lysates. Hela and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14838
Isotype

IgG
Research areas

Western blot - Anti-MCAK antibody [EPR14838] (ab187652)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-MCAK antibody [EPR14838] (ab187652)

Flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
Western blot - Anti-MCAK antibody [EPR14838] (ab187652)

All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Hela cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 81 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)

Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)

Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
Anti-MCAK antibody [EPR14838] (ab187652)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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