Anti-MCAK antibody (ab70536)
Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MCAK antibody
See all MCAK primary antibodies -
Description
Rabbit polyclonal to MCAK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human -
Immunogen
Synthetic peptide corresponding to Human MCAK.
Database link: Q99661 -
Positive control
- IHC: human skin carcinoma and breast carcinoma tissues IP: HeLa cell lysates
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and breast carcinoma (right) tissues labelling MCAK with ab70536 at 1/1000 (1µg/ml). Detection: DAB.
-
MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab70536, ab264148 and ab264149 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 µg/ml.
Lane 1: ab70536 IP in HeLa whole cell lysate.
Lane 2: ab264148 IP in HeLa whole cell lysate.
Lane 3: ab264149 IP in HeLa whole cell lysate.
Lane 4: Control IgG.Exposure time: 10 seconds.
Detection: Chemiluminescence