Anti-MCAK antibody (ab264148)
Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCAK antibody
See all MCAK primary antibodies -
Description
Rabbit polyclonal to MCAK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human MCAK aa 550-600. The exact sequence is proprietary.
Database link: Q99661 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCAK antibody (ab264148)All lanes : Anti-MCAK antibody (ab264148) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-MCAK antibody (ab264148)MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab70536, ab264148 and ab264149 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 µg/ml.
Lane 1: ab70536 IP in HeLa whole cell lysate.
Lane 2: ab264148 IP in HeLa whole cell lysate.
Lane 3: ab264149 IP in HeLa whole cell lysate.
Lane 4: Control IgG.Exposure time: 10 seconds.
Detection: Chemiluminescence
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Immunoprecipitation - Anti-MCAK antibody (ab264148)MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264148 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 µg/ml.
Lane 1: ab264148 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
