Anti-BRF1 antibody (ab264191)
Key features and details
- Rabbit polyclonal to BRF1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BRF1 antibody
See all BRF1 primary antibodies -
Description
Rabbit polyclonal to BRF1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human BRF1 aa 627-677. The exact sequence is proprietary.
Database link: Q92994 -
Positive control
- IHC-P: Human thyroid carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-BRF1 antibody (ab264191)
BRF1 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264191 at 3 µg/mg lysate. ab264191 was used for western blotting at 1 µg/ml.
Lane 1: ab74221 IP in HeLa whole cell lysate.
Lane 2: ab264191 IP in HeLa whole cell lysate.
Lane 3: Control IgG. -
Western blot - Anti-BRF1 antibody (ab264191)All lanes : Anti-BRF1 antibody (ab264191) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (ab264191)Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for BRF1 using ab264191 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
