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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol III Transcription

Anti-BRF1 antibody (ab74221)

Anti-BRF1 antibody (ab74221)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BRF1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BRF1 antibody
    See all BRF1 primary antibodies
  • Description

    Rabbit polyclonal to BRF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, mapping to a region between amino acids 550-600 of Human BRF1 (NP_001510.2)

  • Positive control

    • HeLa or 293T whole cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol III Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (ab74221)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (ab74221)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRF1 with ab74221 at 1/200 (1µg/ml). Detection: DAB.
  • Western blot - Anti-BRF1 antibody (ab74221)
    Western blot - Anti-BRF1 antibody (ab74221)
    All lanes : Anti-BRF1 antibody (ab74221) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 74 kDa
    Observed band size: 74 kDa
    Additional bands at: 140 kDa, 200 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation - Anti-BRF1 antibody (ab74221)
    Immunoprecipitation - Anti-BRF1 antibody (ab74221)
    HeLa whole cell lysate (1mg) was subjected to immunoprecipitation using ab74221 (3µg/mg lysate; lane 1) or a control IgG antibody (lane 2). 20% of the immunoprecipitates were used for Western blotting and bands were identified using ab74221 at a concentration of 1µg/ml. A band at the predicted molecular weight of 74kDa was detected using chemiluminescence with an exposure time of 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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