Anti-POLR3E antibody (ab134560)
Key features and details
- Rabbit polyclonal to POLR3E
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR3E antibody
See all POLR3E primary antibodies -
Description
Rabbit polyclonal to POLR3E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 475-525 of Human POLR3E (NP_060589.1).
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Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLR3E antibody (ab134560)All lanes : Anti-POLR3E antibody (ab134560) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-POLR3E antibody (ab134560)Detection of POLR3E by Western Blot of Immunprecipitate.
Immunoprecipitation of POLR3E in HeLa whole cell lysate, using ab134560 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
For the subsequent WB, ab134560 was used at 1 µg/ml.
The lysate in lane 2 is precipitated using an IgG control antibody.
Detection: Chemiluminescence with an exposure time of 10 seconds.
