Anti-JAK1 antibody (ab47435)
Key features and details
- Rabbit polyclonal to JAK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JAK1 antibody
See all JAK1 primary antibodies -
Description
Rabbit polyclonal to JAK1 -
Host species
Rabbit -
Specificity
Detects endogenous levels of JAK1 protein -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab47435)ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-JAK1 antibody (ab47435)All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size: 132 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab47435)Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
