Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)
  • Suitable for: WB, IHC-P, Dot blot, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)]
    See all JAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)

  • Host species

    Rabbit

  • Specificity

    According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1023, but also recognizes JAK1 with phospho Y1022 if high amount peptides were coated.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human JAK1 aa 1000-1100. The exact sequence is proprietary.
    Database link: P23458

  • Positive control

    • WB: Ramos whole cell lysate. IHC-P: Human colon adenocarcinoma and liver carcinoma tissue.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/5000 dilution

    Lane 1 : Untreated Ramos whole cell lysate
    Lane 2 : Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 133 kDa



    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0.

    PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

    Primary antibody: ab138005 at a 1/1000 dilution (unpurified).

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Lane 1: JAK1 (pY1022) phospho peptide

    Lane 2: JAK1 (pY1023) phospho peptide

    Lane 3: JAK1 (pY1022/pY1023) phospho peptide

    Lane 4: JAK1 non-phospho peptide

    Exposure time: 3 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

    Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/1000 dilution (unpurified)

    Lane 1 : Ramos cell lysates
    Lane 2 : Ramos cell lysates, treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 133 kDa

  • ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

    Antigen:

    1. JAK1 (pY1022) phospho peptide
    2. JAK1 (pY1023) phospho peptide
    3. JAK1 non-phospho peptide

    Antigen concentration: 0~1000ng/ml

    Primary antibody concentration range: 1000ng/ml

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution

     

  • OI-RD Scanning - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    OI-RD Scanning - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

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