Antibodies, Proteins, Kits and Reagents for Life Science

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100 µl 311 587 ₸ 10 µl 127 315 ₸

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Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)
Suitable for: WB, IHC-P, Dot blot, ELISA
Reacts with: Human

Product name

Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)]
See all JAK1 primary antibodies
Description

Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)
Host species

Rabbit
Specificity

According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1023, but also recognizes JAK1 with phospho Y1022 if high amount peptides were coated.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human JAK1 aa 1000-1100. The exact sequence is proprietary.
Database link: P23458
Positive control
- WB: Ramos whole cell lysate. IHC-P: Human colon adenocarcinoma and liver carcinoma tissue.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 1.99 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1899(2)
Isotype

IgG
Research areas

Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/5000 dilution

Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 133 kDa
Observed band size: 133 kDa

Blocking and diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0.

PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Primary antibody: ab138005 at a 1/1000 dilution (unpurified).

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

Lane 1: JAK1 (pY1022) phospho peptide

Lane 2: JAK1 (pY1023) phospho peptide

Lane 3: JAK1 (pY1022/pY1023) phospho peptide

Lane 4: JAK1 non-phospho peptide

Exposure time: 3 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/1000 dilution (unpurified)

Lane 1 : Ramos cell lysates
Lane 2 : Ramos cell lysates, treated with pervanadate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 133 kDa
ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
Antigen:
1. JAK1 (pY1022) phospho peptide
2. JAK1 (pY1023) phospho peptide
3. JAK1 non-phospho peptide
Antigen concentration: 0~1000ng/ml

Primary antibody concentration range: 1000ng/ml

Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution
OI-RD Scanning - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

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