Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAMK1D antibody [EPR3536(2)] (ab172618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3536(2)] to CAMK1D
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CAMK1D antibody [EPR3536(2)]
    See all CAMK1D primary antibodies

  • Description

    Rabbit monoclonal [EPR3536(2)] to CAMK1D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CAMK1D aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IU85

  • Positive control

    • Human fetal forebrain, 293T and HeLa lysates; 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
    Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
    Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
    All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution

    Lane 1 : Human fetal forebrain tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
    Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)

    Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).

     

  • Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
    Anti-CAMK1D antibody [EPR3536(2)] (ab172618)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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