Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
![Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-266712-anti-camk1d-antibody-epr35362-western-blot.jpg "Anti-CAMK1D antibody [EPR3536(2)] (ab172618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3536(2)] to CAMK1D
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CAMK1D antibody [EPR3536(2)]
See all CAMK1D primary antibodies -
Description
Rabbit monoclonal [EPR3536(2)] to CAMK1D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CAMK1D aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IU85 -
Positive control
- Human fetal forebrain, 293T and HeLa lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3536(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-195670-anti-camk1d-antibody-epr35362-western-blot.jpg)
Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution
Lane 1 : Human fetal forebrain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-195671-anti-camk1d-antibody-epr35362-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).
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![Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-4-benefits-of-recombinant-antibodies.png)
Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
