Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10496] to MAT2A + MAT1A
Suitable for: Flow Cyt, WB, ICC
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-MAT2A + MAT1A antibody [EPR10496]
See all MAT2A+MAT1A primary antibodies
Description

Rabbit monoclonal [EPR10496] to MAT2A + MAT1A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
Database link: P31153
Positive control
- ICC: HeLa cells; WB: Human MAT1A/2A recombinant protein, HeLa, HepG2, Ramos, 293T, NIH/3T3 and C6 whole cell lysates; Flow cyt: Ramos and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10496
Isotype

IgG
Research areas

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 44, 46 kDa
why is the actual band size different from the predicted?

MAT1A: 44kDa ; MAT2A: 46kDa

Blocking Buffer and concentration: 5% NFDM/TBST
Immunocytochemistry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:100 dilution (2.52 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Flow Cytometry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution

Lane 1 : Full-length Human MAT1A recombinant protein with His-Tag
Lane 2 : Full-length Human MAT2A recombinant protein with GST-Tag

Lysates/proteins at 0.015 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa

MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house
MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company

Blocking and diluting buffer and concentrations: 5%NFDM/TBST

Exposure time: Lane 1: 1 second
Lane 2: 3 seconds
Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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