Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR349(N)] to JAK1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Anti-CrkL antibody [Y244] (ab32018)
Product image
Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
Product image
Recombinant cynomolgus monkey RIP protein (Active) (ab268943)
Product image
Recombinant Human PCTAIRE2 protein (ab190412)

Overview

  • Product name

    Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free
    See all JAK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR349(N)] to JAK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab170719 is the carrier-free version of ab133666. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab170719 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR349(N)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Images

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)

    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg) 

    Lane 2: Empty

    Lane 3: JAK1 (KO) whole cell lysate (40 µg)

    Lane 4: A431 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133666 observed at 133 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133666 was shown to specifically recognize JAK1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when JAK1 knockout samples were examined. Wild-type and JAK1 knockout samples were subjected to SDS-PAGE. ab133666 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/2000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 133 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified)

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 133 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Western blot - Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (unpurified)

    Lane 1 : Ramos cell lysate
    Lane 2 : HT-29 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 133 kDa



    This data was developed using ab133666, the same antibody clone in a different buffer formulation.

     

  • Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)
    Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-JAK1 antibody [EPR349(N)] - BSA and Azide free (ab170719)

  •  
  • Product image

    Anti-JAK1 antibody (ab47435)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-JAK1 antibody (ab125051)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    HRP Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab215338)

    Applications: Dot, WB

  •  
  • Product image

    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

    Applications: Dot, ELISA, IHC-P, WB

  •  
  • Product image

    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

    Applications: Dot, ELISA, IHC-P, WB

  •  
  • Product image

    Anti-JAK1 antibody [EPR349(N)] (ab133666)

    Applications: WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

  •  
  • Product image

    Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

  •  
  • Product image

    Anti-Galectin 3 antibody [EPR19244] - BSA and Azide free (ab251504)

  •  
  • Product image

    Anti-Actin antibody - Loading Control (ab1801)

  •  
  • Product image

    Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)

  •  
  • Product image

    Anti-beta Amyloid antibody [BAM01], prediluted (ab75046)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.