Anti-JAK1 antibody (ab125051)
Key features and details
- Rabbit polyclonal to JAK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-JAK1 antibody
See all JAK1 primary antibodies -
Description
Rabbit polyclonal to JAK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human JAK1 aa 1100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab140406) -
Positive control
- ICC/IF: HCT116 cells. IHC-P: Human breast fibroadenocarcinoma tissue. WB : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate and HCT 116 (human colorectal carcinoma cell line) whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Western blot - Anti-JAK1 antibody (ab125051)Anti-JAK1 antibody (ab125051) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab125051)ab125051 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125051 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab125051)IHC image of JAK1 staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125051, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
