Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-JAK1 antibody [EPR349(N)] (ab133666)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR349(N)] to JAK1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-JAK1 antibody [EPR349(N)]
See all JAK1 primary antibodies
Description

Rabbit monoclonal [EPR349(N)] to JAK1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human JAK1 (UniProt P23458).
Positive control
- WB: Ramos, HT-29, HeLa and Jurkat whole cell lysate (ab7899)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR349(N)
Isotype

IgG
Research areas

Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: Empty
Lane 3: JAK1 (KO) whole cell lysate (40 µg)
Lane 4: A431 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab133666 observed at 133 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133666 was shown to specifically recognize JAK1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when JAK1 knockout samples were examined. Wild-type and JAK1 knockout samples were subjected to SDS-PAGE. Ab133666 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + rat kidney at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 133 kDa
Observed band size: 130 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/2000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified)

Lane 1 : RAW264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + HeLa at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (unpurified)

Lane 1 : Ramos cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 133 kDa
Anti-JAK1 antibody [EPR349(N)] (ab133666)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com