Anti-14-3-3 beta antibody (ab97273)
Key features and details
- Rabbit polyclonal to 14-3-3 beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 beta antibody
See all 14-3-3 beta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 - 141 of 14-3-3 beta (AAH01359).
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Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates and mouse brain tissue lysate. ICC/IF: HeLa cells. IHC-P: SW480 xenograft and rat cervix tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 beta antibody (ab97273)All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab97273)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SW480 xenograft labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
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Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab97273)ab97273, at a 1/200 dilution, staining 14-3-3 beta in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
Right image is merged with DNA probe. -
Western blot - Anti-14-3-3 beta antibody (ab97273)Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution + Mouse brain tissue lysate at 20 µg
Predicted band size: 28 kDa12% SDS PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab97273)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cervix labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
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Western blot - Anti-14-3-3 beta antibody (ab97273)All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : MOLT4 whole cell lysate
Lane 6 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE
