Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UNC5B antibody [EPR18439] (ab189914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18439] to UNC5B
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Rat

Overview

  • Product name

    Anti-UNC5B antibody [EPR18439]
    See all UNC5B primary antibodies

  • Description

    Rabbit monoclonal [EPR18439] to UNC5B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IP
    Rat
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat cerebral cortex, hippocampus and hypothalamus lysates; C6 whole cell lysate. ICC/IF: C6 cells. IP: Rat cerebral cortex whole cell extract.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UNC5B antibody [EPR18439] (ab189914)
    Western blot - Anti-UNC5B antibody [EPR18439] (ab189914)
    All lanes : Anti-UNC5B antibody [EPR18439] (ab189914) at 1/2000 dilution

    Lane 1 : Rat cerebral cortex lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Rat hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 103 kDa
    Observed band size: 130,60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    UNC5B was cleaved by caspase-3 at Asp412 to produce a cleavage fragment around ~60kDa Llambi F. et al. EMBO J. 2001 Jun 1; 20(11):2715-22.

  • Western blot - Anti-UNC5B antibody [EPR18439] (ab189914)
    Western blot - Anti-UNC5B antibody [EPR18439] (ab189914)
    Anti-UNC5B antibody [EPR18439] (ab189914) at 1/10000 dilution + C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 103 kDa
    Observed band size: 130,60 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    UNC5B was cleaved by caspase-3 at Asp412 to produce a cleavage fragment around ~60kDa Llambi F. et al. EMBO J. 2001 Jun 1; 20(11):2715-22.

  • Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [EPR18439] (ab189914)
    Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [EPR18439] (ab189914)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling UNC5B with ab189914 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm staining on C6 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG(AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab189914 at 1/500 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Immunoprecipitation - Anti-UNC5B antibody [EPR18439] (ab189914)
    Immunoprecipitation - Anti-UNC5B antibody [EPR18439] (ab189914)

    UNC5B was immunoprecipitated from 1mg of Rat cerebral cortex whole cell extract with ab189914 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab189914 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/500 dilution.

    Lane 1: Rat cerebral cortex whole cell extract,10µg (Input).

    Lane 2: ab189914 IP in Rat cerebral cortex whole cell extract.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab189914 in Rat cerebral cortex whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-UNC5B antibody [EPR18439] (ab189914)
    Anti-UNC5B antibody [EPR18439] (ab189914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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