Anti-UNC5B antibody [Guido-1] (ab54430)
![Anti-UNC5B antibody [Guido-1] (ab54430)](https://www.abcam.com/ps/products/54/ab54430/Images/ab54430-67279-anti-unc5b-antibody-guido-1-immunofluorescence.jpg "Anti-UNC5B antibody [Guido-1] (ab54430)")
Key features and details
- Mouse monoclonal [Guido-1] to UNC5B
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-UNC5B antibody [Guido-1]
See all UNC5B primary antibodies -
Description
Mouse monoclonal [Guido-1] to UNC5B -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 28-363 of Human UNC5B
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
Guido-1 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)
ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
