Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4423] to CD33
Suitable for: WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CD33 antibody [EPR4423]
See all CD33 primary antibodies
Description

Rabbit monoclonal [EPR4423] to CD33
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD33 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: THP1 cell lysate, U937 cell lysate, HL 60 cell lysate, HepG2 whole cell lysate (ab7900), HeLa whole cell lysate (ab150035) Flow Cyt: THP1 cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.76 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4423
Isotype

IgG
Research areas

Western blot - Anti-CD33 antibody [EPR4423] (ab134115)

All lanes : Anti-CD33 antibody [EPR4423] (ab134115) at 1/5000 dilution (purified)

Lane 1 : THP-1 cell lysate
Lane 2 : U937 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 67,75 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-CD33 antibody [EPR4423] (ab134115)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP1 (Human monocytic leukemia monocyte, Right) labeling CD33 with ab134115 at 1/500 dilution (red). Goat anti rabbit IgG (Alexa Fluor^® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).

Negative control: Jurkat.
Gated on viable cells.
Immunoprecipitation - Anti-CD33 antibody [EPR4423] (ab134115)

ab134115 (purified) at 1/60 immunoprecipitating CD33 in 10 μg THP-1 (Lanes 1 and 2, observed at 67-75 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
OI-RD Scanning - Anti-CD33 antibody [EPR4423] (ab134115)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Western blot - Anti-CD33 antibody [EPR4423] (ab134115)

All lanes : Anti-CD33 antibody [EPR4423] (ab134115) at 1/1000 dilution (unpurified)

Lane 1 : THP1 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 67-75 kDa why is the actual band size different from the predicted?
Anti-CD33 antibody [EPR4423] (ab134115)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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