Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD33 antibody [EPR4423] (ab134115)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4423] to CD33
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD33 antibody [EPR4423]
    See all CD33 primary antibodies

  • Description

    Rabbit monoclonal [EPR4423] to CD33

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD33 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: THP1 cell lysate, U937 cell lysate, HL 60 cell lysate, HepG2 whole cell lysate (ab7900), HeLa whole cell lysate (ab150035) Flow Cyt: THP1 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CD33 antibody [EPR4423] (ab134115)
    Western blot - Anti-CD33 antibody [EPR4423] (ab134115)
    All lanes : Anti-CD33 antibody [EPR4423] (ab134115) at 1/5000 dilution (purified)

    Lane 1 : THP-1 cell lysate
    Lane 2 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 67,75 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-CD33 antibody [EPR4423] (ab134115)
    Flow Cytometry - Anti-CD33 antibody [EPR4423] (ab134115)
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP1 (Human monocytic leukemia monocyte, Right) labeling CD33 with ab134115 at 1/500 dilution (red). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).
     
    Negative control: Jurkat.
    Gated on viable cells.

     

  • Immunoprecipitation - Anti-CD33 antibody [EPR4423] (ab134115)
    Immunoprecipitation - Anti-CD33 antibody [EPR4423] (ab134115)

    ab134115 (purified) at 1/60 immunoprecipitating CD33 in 10 μg THP-1 (Lanes 1 and 2, observed at 67-75 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

  • OI-RD Scanning - Anti-CD33 antibody [EPR4423] (ab134115)
    OI-RD Scanning - Anti-CD33 antibody [EPR4423] (ab134115)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-CD33 antibody [EPR4423] (ab134115)
    Western blot - Anti-CD33 antibody [EPR4423] (ab134115)
    All lanes : Anti-CD33 antibody [EPR4423] (ab134115) at 1/1000 dilution (unpurified)

    Lane 1 : THP1 cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 67-75 kDa why is the actual band size different from the predicted?

  • Anti-CD33 antibody [EPR4423] (ab134115)
    Anti-CD33 antibody [EPR4423] (ab134115)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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