Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
![Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)](https://www.abcam.com/ps/products/6/ab6083/Images/ab6083-199461-anti-cd14-antibody-mem-18-flow-cytometry.jpg "Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)")
Key features and details
- Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free
See all CD14 primary antibodies -
Description
Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MEM-18 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
