Anti-CD14 antibody (ab185538)
Key features and details
- Rabbit polyclonal to CD14
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD14 antibody
See all CD14 primary antibodies -
Description
Rabbit polyclonal to CD14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- RAW 264.7, NIH/3T3 and SW480 cell lysates; PNGase F treated SW480 lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD14 antibody (ab185538)All lanes : Anti-CD14 antibody (ab185538) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?The higher observed MW is due to the glycosylation of CD14.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-CD14 antibody (ab185538)All lanes : Anti-CD14 antibody (ab185538) at 1/200 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) control lysate
Lane 2 : PNGase F treated SW480 (Human colon adenocarcinoma cell line) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39,55 kDa why is the actual band size different from the predicted?The higher observed MW is due to the glycosylation of CD14.
Blocking/Dilution buffer: 5% NFDM/TBST.
