Anti-CD33 antibody [SP266] (ab199432)
![Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-14-AntiCD33-antibody-SP266-IHC-human-placenta.jpg "Anti-CD33 antibody [SP266] (ab199432)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP266] to CD33
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-CD33 antibody [SP266]
See all CD33 primary antibodies -
Description
Rabbit monoclonal [SP266] to CD33 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP1 cell lysate. IHC-P: Human tonsil, lung, colon, thymus, liver, placenta, spleen, bladder cystitis, skin squamous cell carcinoma, stomach adrenocarcinoma and renal cell carcinoma tissues.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP266 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling CD33 with ab199432 at 1/100 dilution (5.81 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human placenta, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab199432 for 10 mins at room temperature. -
![Western blot - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238715-anti-cd33-antibody-sp266-western-blot.jpg)
Western blot - Anti-CD33 antibody [SP266] (ab199432)Anti-CD33 antibody [SP266] (ab199432) at 1/100 dilution + THP1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 40 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238716-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD33 using ab199432 at a 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238717-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238718-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238719-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human thymus tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238720-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238721-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238723-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder cystitis tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238724-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238725-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-238726-anti-cd33-antibody-sp266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [SP266] (ab199432)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinoma tissue labeling CD33 using ab199432 at 1/100 dilution.
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![Anti-CD33 antibody [SP266] (ab199432)](https://www.abcam.com/ps/products/199/ab199432/Images/ab199432-16-benefits-of-recombinant-antibodies.png)
Anti-CD33 antibody [SP266] (ab199432)
