Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JAML antibody [EPR15289] - BSA and Azide free
    See all JAML primary antibodies

  • Description

    Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6 and RAW 264.7 whole cell lysates.

  • General notes

    Ab232632 is the carrier-free version of ab183714. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232632 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
    Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)

    Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

  • Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
    Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
    All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution

    Lane 1 : C6 (rat glioma cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

  • Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
    Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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