Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
![Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-2-ab183714216194Picture8.jpg "Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-JAML antibody [EPR15289] - BSA and Azide free
See all JAML primary antibodies -
Description
Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6 and RAW 264.7 whole cell lysates.
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General notes
Ab232632 is the carrier-free version of ab183714. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232632 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15289 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).
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![Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-308791-anti-jaml-antibody-epr15289-western-blot.jpg)
Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution
Lane 1 : C6 (rat glioma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).
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![Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-3-benefits-of-recombinant-antibodies.png)
Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
