Anti-JAML antibody [EPR15289] (ab183714)
![Anti-JAML antibody [EPR15289] (ab183714)](https://www.abcam.com/ps/products/183/ab183714/Images/ab183714-216194-anti-jaml-antibody-epr15289-immunoprecipitation.jpg "Anti-JAML antibody [EPR15289] (ab183714)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15289] to JAML
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-JAML antibody [EPR15289]
See all JAML primary antibodies -
Description
Rabbit monoclonal [EPR15289] to JAML -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB RatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, Jurkat, Molt-4, 293, C6 and Raw264.7 cell lysates; Molt-4 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15289 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-JAML antibody [EPR15289] (ab183714)
Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
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![Western blot - Anti-JAML antibody [EPR15289] (ab183714)](https://www.abcam.com/ps/products/183/ab183714/Images/ab183714-216192-anti-jaml-antibody-epr15289-western-blot.jpg)
Western blot - Anti-JAML antibody [EPR15289] (ab183714)All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-JAML antibody [EPR15289] (ab183714)](https://www.abcam.com/ps/products/183/ab183714/Images/ab183714-216191-anti-jaml-antibody-epr15289-western-blot.jpg)
Western blot - Anti-JAML antibody [EPR15289] (ab183714)All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/20000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Anti-JAML antibody [EPR15289] (ab183714)](https://www.abcam.com/ps/products/183/ab183714/Images/ab183714-5-benefits-of-recombinant-antibodies.png)
Anti-JAML antibody [EPR15289] (ab183714)
