Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP7235] to EPM2A/Laforin
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-EPM2A/Laforin antibody [EP7235]
See all EPM2A/Laforin primary antibodies
Description

Rabbit monoclonal [EP7235] to EPM2A/Laforin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human EPM2A/Laforin aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- Human fetal heart and muscle, HeLa, and 293T cell lysates; Human kidney and muscle tissues; HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as EPM2A', 'EPM2A, isoform 9

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP7235
Isotype

IgG
Research areas

Western blot - Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

All lanes : Anti-EPM2A/Laforin antibody [EP7235] (ab129110) at 1/100 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EPM2A/Laforin with purified ab129110 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human muscle tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

ab129110, at 1/100 dilution, staining EPM2A/Laforin in HeLa cells by Immunofluorescence.
Anti-EPM2A/Laforin antibody [EP7235] (ab129110)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com