Anti-EPM2A/Laforin antibody [EP7235] (ab129110)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7235] to EPM2A/Laforin
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-EPM2A/Laforin antibody [EP7235]
See all EPM2A/Laforin primary antibodies -
Description
Rabbit monoclonal [EP7235] to EPM2A/Laforin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human EPM2A/Laforin aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Human fetal heart and muscle, HeLa, and 293T cell lysates; Human kidney and muscle tissues; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as EPM2A', 'EPM2A, isoform 9
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7235 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EPM2A/Laforin antibody [EP7235] (ab129110) at 1/100 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
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ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EPM2A/Laforin with purified ab129110 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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ab129110, at 1/100 dilution, staining EPM2A/Laforin in Paraffin-embedded Human muscle tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab129110, at 1/100 dilution, staining EPM2A/Laforin in HeLa cells by Immunofluorescence.
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