Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)
![Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)](https://www.abcam.com/ps/products/215/ab215383/Images/ab215383-296610-anti-cd33-antibody-epr4423-.jpg "Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4423] to CD33 - Low endotoxin, Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free
See all CD33 primary antibodies -
Description
Rabbit monoclonal [EPR4423] to CD33 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
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General notes
ab215383 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.76 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4423 -
Isotype
IgG -
Research areas
Images
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OI-RD Scanning - Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Immunoprecipitation - Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)](https://www.abcam.com/ps/products/215/ab215383/Images/ab215383-296609-anti-cd33-antibody-epr4423-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)This IP data was generated using the same anti-CD33 antibody clone, EPR4423, in a different buffer formulation (cat# ab134115).
ab134115 (purified) at 1/60 immunoprecipitating CD33 in 10 μg THP-1 (Lanes 1 and 2, observed at 67-75 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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![Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)](https://www.abcam.com/ps/products/215/ab215383/Images/ab215383-3-benefits-of-recombinant-antibodies.png)
Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (ab215383)
