Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR684(2)] to Fibulin-4
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Fibulin-4 antibody [EPR684(2)]
    See all Fibulin-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR684(2)] to Fibulin-4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Fibulin-4 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • JAR and SW480 cell lysates, Human fetal lung lysate, Human breast carcinoma and muscle tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as EFEMP2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR684(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) whole cell lysates
    Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysates
    Lane 3 : Human fetal lung lysates
    Lane 4 : Human colon cancer lysates
    Lane 5 : Human placenta lysates
    Lane 6 : Mouse placenta lysates
    Lane 7 : Mouse lung lysates
    Lane 8 : NIH/3T3 (mouse embryo) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 49 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Human fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)

    ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)

    ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
    Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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