Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
![Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-252270-anti-efemp2-antibody-epr6842-western-blot.jpg "Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR684(2)] to Fibulin-4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Fibulin-4 antibody [EPR684(2)]
See all Fibulin-4 primary antibodies -
Description
Rabbit monoclonal [EPR684(2)] to Fibulin-4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human Fibulin-4 aa 400-500. The exact sequence is proprietary.
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Positive control
- JAR and SW480 cell lysates, Human fetal lung lysate, Human breast carcinoma and muscle tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as EFEMP2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR684(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) whole cell lysates
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysates
Lane 3 : Human fetal lung lysates
Lane 4 : Human colon cancer lysates
Lane 5 : Human placenta lysates
Lane 6 : Mouse placenta lysates
Lane 7 : Mouse lung lysates
Lane 8 : NIH/3T3 (mouse embryo) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139220-anti-efemp2-antibody-epr6842-western-blot.jpg)
Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139219-anti-efemp2-antibody-epr6842-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139218-anti-efemp2-antibody-epr6842-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-5-benefits-of-recombinant-antibodies.png)
Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
