Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-YKL-40/CHI3L1 antibody [EPR23891-162]
    See all YKL-40/CHI3L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen and lung tissue lysate. IHC-P: Mouse lung, spleen and colon cancer tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23891-162

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse lung tissues (PMID:26528713). The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
    All lanes : Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:

    Lane 1: 81 seconds

    Lane 2: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse spleen tissue. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)

    Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on immune cells of mouse colon cancer tissues. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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