Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
![Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-1-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-lung.jpg "Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Overview
-
Product name
Anti-YKL-40/CHI3L1 antibody [EPR23891-162]
See all YKL-40/CHI3L1 primary antibodies -
Description
Rabbit monoclonal [EPR23891-162] to YKL-40/CHI3L1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse spleen and lung tissue lysate. IHC-P: Mouse lung, spleen and colon cancer tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23891-162 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse lung tissues (PMID:26528713). The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-448946-anti-ykl40chi3l1-antibody-epr23891162-western-blot-lysate.jpg)
Western blot - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)All lanes : Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDa
Observed band size: 43 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 81 seconds
Lane 2: 10 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-2-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse spleen tissue. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)](https://www.abcam.com/ps/products/259/ab259322/Images/ab259322-3-anti-ykl40chi3l1-antibody-epr23891162-immunohistochemistry-mouse-colon-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR23891-162] (ab259322)Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling YKL-40/CHI3L1 with ab259322 at 1/800 (0.62 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on immune cells of mouse colon cancer tissues. The section was incubated with ab259322 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins