Human P-cadherin ELISA Kit (CDH3) (ab274395)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 77.07 pg/ml
- Range: 234.38 pg/ml - 15000 pg/ml
- Sample type: EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human P-cadherin ELISA Kit (CDH3)
See all P cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.3% Inter-assay Sample n Mean SD CV% Serum 3 7.5% -
Sample type
Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
77.07 pg/ml -
Range
234.38 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109 101% - 123% Hep Plasma 94 89% - 97% EDTA Plasma 96 93% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human P-cadherin ELISA kit (ab274395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human P-caderin protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human P-cadherin with 77.07 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
P-cadherin (CDH3) is a member of the cadherin family of calcium-dependent adhesion proteins. P-cadherin is expressed in epithelial cells and some cancers. Soluble P-cadherin expression is correlated with tumor stage in non-invasive lung cancer and is a target for potential therapeutic treatment of cancer.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human P-cadherin (CDH3) Capture Antibody 1 x 600µl 10X Human P-cadherin (CDH3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human P-cadherin (CDH3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Expressed in some normal epithelial tissues and in some carcinoma cell lines. -
Involvement in disease
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CADH3_HUMAN
- Cadherin 3
- Cadherin 3 precursor
see all -
Database links
- Entrez Gene: 1001 Human
- Omim: 114021 Human
- SwissProt: P22223 Human
- Unigene: 191842 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human P-cadherin standard curve in Sample Diluent 25BS.The P-cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native P-cadherin in human serum and plasma samples.The concentrations of P-cadherin were measured in duplicates, interpolated from the P-cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean P-cadherin concentration was determined to be 9,438 pg/mL in serum, 7,171 pg/mL in plasma (heparin), and 5,427 pg/mL in plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean P-cadherin concentration was determined to be 7,429 pg/mL with a range of 4,127 – 9,721 pg/mL.
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Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (ab274395)To learn more about the advantages of recombinant antibodies see here.
