Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)
![Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.com/ps/products/12/ab12077/Images/ab12077-336311-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg "Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)")
Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1
- Suitable for: IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Desmoglein 1/DSG1 antibody [27B2]
See all Desmoglein 1/DSG1 primary antibodies -
Description
Mouse monoclonal [27B2] to Desmoglein 1/DSG1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- IHC-Fr: Human normal skin.
-
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product was previously labelled as Desmoglein 1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
27B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)
IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
-
![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.com/ps/products/12/ab12077/Images/ab12077-19418-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077) This image is courtesy of an anonymous Abreview.ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.
