Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
![Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)](https://www.abcam.com/ps/products/209/ab209490/Images/ab209490-395639-AntiDesmoglein-1-DSG1-antibody.jpg "Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Desmoglein 1/DSG1 antibody [EPR20382]
See all Desmoglein 1/DSG1 primary antibodies -
Description
Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skin tissue lysate. IHC-P: Human esophagus and skin tissue.
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General notes
This product was previously labelled as Desmoglein 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20382 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)All lanes : Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490) at 1/5000 dilution
Lane 1 : Human skin lysates prepared in RIPA lysis method
Lane 2 : Human skin lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG
Predicted band size: 114 kDaThe expression profile and molecular weight observed are consistent with what have been described in the literature (PMID: 10634593, 29192645)
Observed MW: 150kDa
Block/diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)](https://www.abcam.com/ps/products/209/ab209490/Images/ab209490-311767-anti-desmoglein-1-antibody-epr20382-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)](https://www.abcam.com/ps/products/209/ab209490/Images/ab209490-311766-anti-desmoglein-1-antibody-epr20382-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous staining on human esophagus (PMID: 24220297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
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![Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)](https://www.abcam.com/ps/products/209/ab209490/Images/ab209490-5-benefits-of-recombinant-antibodies.png)
Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
