Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Desmoglein 1/DSG1 antibody [EPR20382]
See all Desmoglein 1/DSG1 primary antibodies
Description

Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skin tissue lysate. IHC-P: Human esophagus and skin tissue.
General notes
This product was previously labelled as Desmoglein 1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20382
Isotype

IgG
Research areas

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

All lanes : Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490) at 1/5000 dilution

Lane 1 : Human skin lysates prepared in RIPA lysis method
Lane 2 : Human skin lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

Predicted band size: 114 kDa

The expression profile and molecular weight observed are consistent with what have been described in the literature (PMID: 10634593, 29192645)

Observed MW: 150kDa

Block/diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous staining on human esophagus (PMID: 24220297) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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