Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free
    See all Vitronectin/S-Protein primary antibodies

  • Description

    Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human serum.

  • General notes

    ab247306 is the carrier-free version of ab46808 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247306 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution + Human serum at 10 µg

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab46808, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    This data was developed using ab46808, the same antibody clone in a different buffer formulation.This image shows human lung tissue treated with ab46808 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)

    This data was developed using ab46808, the same antibody clone in a different buffer formulation.
    Purified ab46808 at 1/50 dilution (2µg) immunoprecipitating Vitronectin/S-Protein in Human serum.
    Lane 1 (input): Human serum 10µg
    Lane 2 (+): ab46808 + Human serum.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 65-75 kDa

  • Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)
    Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (ab247306)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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