Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free
    See all Vitronectin/S-Protein primary antibodies

  • Description

    Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue. ICC: HepG2 cells

  • General notes

    ab271837 is the carrier-free version of ab45139. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)

    Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45139).
  • Immunocytochemistry - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)
    Immunocytochemistry - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)
    This data was developed using the same antibody clone in a different buffer formulation (ab45139).
    Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)
    Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (ab271837)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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