Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [EP781Y]
    See all Vitronectin/S-Protein primary antibodies

  • Description

    Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Vitronectin/S-Protein aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Human serum IHC-P: Human lung tissue. IP: Human serum

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.

  • Clonality

    Monoclonal

  • Clone number

    EP781Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
    Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
    Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution + Human serum at 10 µg

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 54 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

    This image shows human lung tissue treated with ab46808 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
    Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

    Purified ab46808 at 1/50 dilution (2µg) immunoprecipitating Vitronectin/S-Protein in Human serum.
    Lane 1 (input): Human serum 10µg
    Lane 2 (+): ab46808 + Human serum.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 65-75 kDa

  • Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
    Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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