Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Vitronectin/S-Protein antibody [EP873Y]
See all Vitronectin/S-Protein primary antibodies -
Description
Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Vitronectin/S-Protein aa 450-550. The exact sequence is proprietary.
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Positive control
- IHC-P: Human liver tissue. WB: Human serum tissue lysate ICC: HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP873Y -
Isotype
IgG -
Research areas
Images
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Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution + Human serum tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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