Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µl 308 236 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
Suitable for: ICC, WB, IHC-P
Reacts with: Human

Product name

Anti-Vitronectin/S-Protein antibody [EP873Y]
See all Vitronectin/S-Protein primary antibodies
Description

Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Vitronectin/S-Protein aa 450-550. The exact sequence is proprietary.
Positive control
- IHC-P: Human liver tissue. WB: Human serum tissue lysate ICC: HepG2 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP873Y
Isotype

IgG
Research areas

Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution + Human serum tissue lysate at 20 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds
Immunocytochemistry - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com